Effect of (BA, 2,4-D) on induction of callus from Moringa oleifera L. in vitro

The experiment was conducted in the tissue culture laboratory of Plant Production Department - Al-Mussaib Technical College Al Furat Awsat University for period from (1-11-2021 to 1-2-2022). In order study effect growth regulators BA at concentrations (0-0.5-1-1.5-2) mg/L and combination with 2,4-D on induction callus resulting plant parts (Apical Tips new shoots) taken Moringa oleifera L.) invitro.The results showed following: sterilization gave highest a concentration 40% chlorax solution, time 10 minutes, percentage contamination 0%. interaction between auxin cytokinin significant dry weight callus, treatment 5 2 0.5 excelled giving that 0.11 mg/L. lowest is when control x if it 0.01 fresh 4 1.5 reached 1.45 mg /L 0 2,4-D×BA, where 1.04